Liquid Chromatography for Proteins and Peptides
The separation of tissue and blood plasma derived proteins and peptides is a key method in our lab. In particular, size exclusion chromatography is a method we frequently employ for size/shape-based separations with automated fraction collection. Collected fractions may then be further analysed either directly by LC-MS/MS, or after digestion of proteins to peptides.
Columns for Chromatography
For reverse-phase separations of peptide mixtures with online coupling to a mass spectrometer via electrospray ionisation (ESI), we typically use self-packed columns. These columns are packed into fused-silica pulled-tip emitters of internal diameters from 75 um (nanoflow <500 nL/min), up to 150 um (capillary flow <10 uL/min).